Biological Specimens in Electron Microscopes 2025
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Information
Biology Centre of CAS – Institute of Parasitology and Faculty of Science – Institute of Physics and Biophysics, University of South Bohemia, České Budějovice, Czech Republic
This practical course is focused on the advanced methods of biological specimen preparation for electron microscopy. The main goal is to make participants familiar with the theoretical background and the latest practical developments in this field. The programme includes state of the art fixation, including cryo-methods, cutting of ultrathin sections from embedded or frozen samples, methods of negative staining and immunolabeling, specimen preparation for transmission and scanning electron microscopy and methods of specimen preparation for 3D electron microscopy (single particle analysis, electron tomography, array tomography and serial block-face). The results will be evaluated using electron microscopes. The course is limited to 15 participants. It is intended for Master and PhD students, young researchers, technicians, and new EM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging)“.
Organizers & Speakers
Marie Vancová
Since 2021, the head of the LEM. Electron microsocpy of biological specimens, Sample preparation, Cryo sample preparation, Sectioning, Cryo sectioning, Immunolocalization,
Jana Nebesářová
The former head of LEM. Electron microscopy, Specimen preparation, Sectioning, Biological Imaging
Vladislav Krzyžánek
President of EMS, Electron Microscopy, Biomedical Imaging, STEM imaging, Quantitative imaging
Jiří Týč
Volume-EM specialist, SBF-SEM, Arry tomography, Confocal microscopy, Bioimaging
Zdeno Gardian
SPA specialist, TEM, SPA, Image processing, Structural biology
Tomáš Bílý
TEM-tomography specialist, Image processing 3D visualization
František Kitzberger
EM-Image processing/segmentation specialist, Volume SEM, Image processing, segmentation, analysis
Programme
23 - 27 June 2025, České Budějovice
| Monday | ||
| 13:00 | Welcome Address | Marie Vancová |
| 13:10 | Principles of Transmission Electron Microscopy | Tomáš Bílý |
| 14:00 | Overview of TEM Sample Preparation Methods | Jana Nebesářová |
| 14:45 | Coffee break | |
| 15:05 | How to visualize macromolecules in TEM | Zdeno Gardian |
| 16:00 | Room temperature sectioning | Jana Nebesářová |
| Tuesday | ||
| 8:30 | Cryo Methods in Sample Preparation | Marie Vancová |
| 9:15 | Principles of Scanning Electron Microscopy | Vladislav Krzyzanek |
| 10:00 | Coffee break | |
| 10:20 | Immunogold Labeling | Marie Vancová |
| 11:15 | Specimen Preparation for SEM | Jana Nebesářová |
| 12:00 | Lunch | |
| 13:00 | Practicals | |
| Wednesday | ||
| 8:30 | Electron Tomography | Tomáš Bílý |
| 9:30 | Volume SEM techniques (SBF-SEM, Array Tomography) | Jiří Týč |
| 10:20 | Coffee Break | |
| 10:40 | Introduction to Image Processing | František Kitzberger |
| 13:10 | Cellular ultrastructure and interpretation of images | Marie Vancová |
| 12:10 | Lunch | |
| 13:00 | Practicals | |
| Thursday | ||
| 8:30 | Practicals | |
| 12:00 | Lunch | |
| 13:00 | Practicals | |
| Friday | ||
| 8:30 | Practicals | |
| 12:00 | Lunch | |
| 13:00 | Round table discussion | |
Registration
Registration Fees:
CSMS member: 6000 CZK
CSMS non-member: 7000 CZK
Industry: 10000 CZK
The course fee includes admission, the course materials, meals and coffee breaks.
For registration click here
Contact: Martina Tesařová (holland@paru.cas.cz)
Venue and accomodation
Venue: Laboratory of Electron Microscopy, Biology Centre CAS, České Budějovice.
Branišovská 31, 370 05, České Budějovice, Czech Republic
(See map below)
How to get to the venue
By public transport:
From the train station of the bus station, go the bus stop located under the Mercury shopping centre and take bus No. 3 in direction Máj – Antonína Barcala and get off at the bus stop Jihočeská Univerzita. Then cross the street to the university campus and from go to the building of the laboratory.
By car:
In case you plan to get here by the car, go to the address: Branisovska 31, 370 05, České Budejovice. You can park your car on the parking lot at the Biology Centre. (ring at the gate and tell that you participate on this course if asked). Other parking possibility is at the recomended accommodation site.
Accommodation
You can arrange your accommodation either in the Penzion U Šípku (contact person: Marie Hloušková, +420 604 375 441, usipku.cb@seznam.cz), or in the Penzion Dvořák (+420 721 810 410, PenzionDvorak@email.cz). Both of the pensions are located within 15 minutes of walk from the venue. For closer information see the map.
