RNDr. Eva Horáková, Ph.D.
Vědecký pracovník - Laboratoř molekulární biologie prvoků
Molekulární parazitologie
Parazitologický ústav
+420 387775481
horakova@paru.cas.cz
PARU II, 1. patro
EDUCATION 2000-2005 Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic Ph.D. In Molecular Biology Thesis :“Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics.“ supervisor: Prof. Julius Lukes, email: jula@paru.cas.cz 1995-2000 Faculty of Science, Masaryk University, Brno, Czech Republic M.S. In Molecular Biology and Genetics Thesis: „Typization of HLA antigens in CVID patients“ supervisor: MUDr. Tomas Freiberger Ph.D., email: tomf@vuzd.cz WORK EXPERIENCE 2010-present Postdoctoral Fellow, Laboratory of Molecular Parasitology, Institute of Parasitology, Biology Centre, ASCR, v.v.i. „Posttranscriptional modifications of mitochondrial and cytosolic tRNAs in Trypanosoma brucei“ 2009 – 2010 Postdoctoral Fellow, McMaster laboratory, Department of Medical Genetics, Vancouver Coastal Health Research Institute, University of British Columbia, Jack Bell Research Centre “Proteomic Profiling of Host-Pathogen interaction: The Macrophage response to Leishmania major” Prof. Robert W. McMaster (robm@interchange.ubc.ca) 2006-2008 Maternity leave 2000-2005 Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic. Research Scientist in Laboratory of Molecular Parasitology 2003 6 month research stay in Prof. Kenneth Stuart laboratory, Seattle, Biomedical Research Institute, Seattle, Washington, USA 2002 6 month research stay in Dr. Rob Benne laboratory, Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, supported by EURESCO Exchange Grant given to E.V. 1999-2000 Department of Imunogenetics, Research Institute of Child Health, Brno, Czech Republic bachelor Student of Molecular Biology and Genetics Notable awards: 2007, Otto Wichterle Premium for young scientific team, AS CR 2005, honored PhD thesis “Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics” 2001, honored student grant “RNA interference in Trypanosoma brucei” ACADEMICAL COURSES “Grant Writing for Funding and Research Development” University of British Columbia “Mass Spectrometry in Life Sciences,” University of British Columbia “Proactive Communication & Effective Temwork Workshop” MITACS Skills Training&Entrepreneurship Program (STEP) SUPERVISION OF UNDERGRADUATE STUDENTS 2010 Melanie van Soeren: Characterization of the Leishmania protein GP63 using site direct mutagenesis LABORATORY SKILLS Wide range of general lab experience, focusing on molecular biology and proteomics: Culture work (Leishmania ssp, Trypanosoma ssp.), Culture manipulation, Transfection studies, Macrophage infection studies, THP-1 cell cultivation, Peritoneal macrophage cell harvest. Isolation of amastigotes from spleen/lesion, axenic cultivation of amastigotes Leishmaniainfection rate. Limited Dilution assay. Spleen/Liver smears (H/E staining) Viability assays (MTS assay, Alamar Blue assay) Mass spectrometry sample preparation. Operating ABI 4800 mass spec, Protein Pilot, Statistical analysis of proteomics data. Site-direct mutagenesis Gelatin zymography FACS analysis. Isolation of DNA, RNA and mitochondrial vesicles gel electrophoresis, SDS-PAGE,, 2D gel electrophoresis Western and Northern blotting Radiolabelling, Imunochemical staining RNA interference of Trypanosoma brucei immunofluorescent microscopy PCR, RT-PCR, Quantitative RT-PCR, poisoned primer extension in vitro editing measurement of mitochondrial membrane potential measurement of respiration rate histochemical staining of protein complexes NADH dehydrogenase, cytochrome creductase, and oxidase activity assays
