Explanation of some terms
Plunge Freezing - vitrification of thin layer of sample on the TEM grid using liquid ethane. (up to 100 nm)
HPF/High-Pressure Freezing - Sample vitrification using pressurized liquid nitrogen - 3 mm or 6 mm targets, max depth 200 µm; cell monolayer vitrification on the sapphire discs
AFS/Automatic Freeze Substitution equipped with a unit for the UV polymerization - Sample preparation (fixation, embedding, and polymerization) at very low temperatures.
Ultramicrotomes - preparation of semithin and ultrathin sections of samples embedded in the resin at room temperature, the possibility of preparation of serial sections and SBF-SEM pyramids, the possibility of the sample preparation in cryo-conditions (Tokuysu)
Carbon coating of the sample - Sample on the TEM grid, coating, shading, glow discharge
Sputter coating of the sample - Au, Pt, Pt/Pd
Plasma sample cleaning
CPD - Critical point drying of the SEM samples
Glass knife making using the balance breaking method
