Microscopes and data-processing workstations
Transmission Electron Microscopes
JEM-1400 JEOL
- Accelerating voltage: 80 - 120 kV
- Cathode - Tungsten Filament or LaB6
- Resolution: 0.38 nm (point image)
- Image acquisition: CMOS camera XAROSA (EMSIS GmbH) 20MP
- Vacuum: 10-5 Pa
- Cryo Transfer Tomography Holder 2550
- High-tilt tomography holder up to 80 deg
- JEOL Specimen quartet holder
- Software for Stitching and correlative microscopy
- Contact person: Vaněček Jiří
- Booking
JEM–2100F JEOL
- Accelerating voltage: 200 kV
- Optional: 120 kV and 100 - 80 kV
- Cathode: Schottky emission (W-ZrO)
- Resolving power: TEM – point 0.27 nm, lattice 0.14 nm
- Image capture: GATAN K2 Summit direct detector 3838x3710, Super-resolution 7676x7420
- Linear mode, counting mode with drift correction
- JEOL Phase Plate (VOLTA)
- Tomography cryo holder Elsa 698 with temperature controller
- Cryo-transfer workstation
- Software for automated single particle analysis imaging
- Motorized stage with piezoelectric movement
- SerialEM tomography software
- Vacuum: 10-8 Pa
- Solarus II advanced plasma system (pumping station with plasma cleaner Ar+O2)
- Contact person: Bílý Tomáš Ph.D., Gardian Zdenko, Ing. Ph.D.
Scanning electron microscopes
JSM-7401F JEOL
- Accelerating voltage: 0.1 - 30 kV
- Cathode: Field emission (W - single crystal)
- Resolving power: 1 nm
- Image capture: 1280×1024×8bit (with averaging 10bit)
- Cryo attachment: Cryo-ALTO 2000 (GATAN) allows:
- Sample freezing (possible transfer from HPF or Plunge Freezer)
- Freeze fracturing
- Feeze sublimation and sputter coating Au, Pt/Pd
- Vacuum: 10-7 Pa
- Contact person: Vaněček Jiří
- Booking
Apreo ThermofisherScientific
- Accelerating voltage: 0.2 – 30 kV
- Electron landing energy: 20 eV – 30 keV
- Cathode: Field emission (Schottky)
- Resolving power: 0.7nm @15kV, 0.8nm @1kV, 0.9nm @500V, STEM 0.8nm @30kV, LowVac 1.8nm @3kV
- Image capture: up to 6144 x 4096 px
- Vacuum: HiVac 10-2 – 10-4 Pa, LowVac 10–500 Pa
- Stage: high tilt range +-90 degrees
- Extension: Serial Block Face using VolumeScope, Array tomography, Stitching software (MAPS), STEM holder for 6 TEM grids, Amira visualization software
- Contact person:Týč Jiří, RNDr. Ph.D.
Light Microscope
Nikon Eclipse Ts2FL - OTUS
- Optical, fluorescence, inverted mikcroscope
- Diascopic imaging + epifluorescence
- High sensitivity monochromatic camera (QE 82%)
- NIS Elements - imaging and analysis software
- multi-channel fluorescence, cell counting, cell shape analysis, fluorescence intensity.
- Objectives Plan Fluor 4x, 10x a 40x
- Filter cubes: excitation 538-580nm/emission 590-670nm; excitation 500-550nm/emission 565-635nm
