Diversity of soil microbial community in process of primary succession on post-brown-coal-mining sites

 Dana Elhottová, Stanislav Malý, Václav Krištůfek, Alica Chroňáková, Jan Frouz, Jiří Kalčík

New knowledge about qualitative and quantitative changes of soil microbial community in different stage of primary succession on non reclaimed post-brown-coal-mining sites (Velká podkrušnohorská výsypka, Sokolov coal-mining district) were acquired in three-years research. The active pristine as well as died fossil microbial community of freshly exposed spoil substrate (Miocene non toxic lacustrine sediment - Cypris serie) and consequent succession changes of the community in initial (0- 3 years), early (10-12 y.), mid (20-22 y.) and late (42-44 y.) stage were described. The composition of bacterial community in the initial stage indicated an occurrence of species important for biotechnology application. A fundamental qualitative community shift was found ten years after heaping the spoil substrate and consisted in increasing of structural, functional and species diversity. Original results were showed by comparison of community succession changes in surface (0-5 cm) and mineral (10-15 cm) layer. The most important environmental factors responsible for these changes were determined. The community succession patterns exerting in the studied soil ecosystem were designed. The other project gains consisted in the enrichment of methodology suitable for qualitative study of the soil microbial community in situ.