Preparation of capsid protein constructs from GFLV based on local isolates

Coat protein genes of grapevine fanleaf virus local strains isolated in South-Moravia, Czech Republic were sequenced, and artificial coat protein gene was designed and synthesized that retains typical sequence features of local strains but is free of mRNA destabilizing sequences. Three variants of the synthetic gene were cloned into Agrobacterium plant expression vector and their function was tested after potato protoplasts transformation, assaying transient mRNA and coat protein production. Vitis vinifera rootstock cultivars were transformed with Agrobacterium tumefaciens LBA4404 carrying vectors with variants of the synthetic gene, using the meristematic bulk tissue method.

• Vlasák Josef, Bříza Jindřich, Niedermeierová Hana, Pavingerová Dana, Eichmeier A., Baránek M., Pidra M.:GFLV coat protein constructs based on local isolates from the Czech Republic. African Journal of Biotechnology 11(5): 1133-1137, 2012 .-> Abstract / Full-text online