Mgr. Hana Tykalová, Ph.D.

Professional experience

2022 – present        R&D Scientist, Institute of Parasitology, Biology Centre, Czech Academy of Sciences

2019 – present        Research Assistant, Faculty of Science, University of South Bohemia in České Budějovice, Laboratory of

                                Applied Biochemistry

2013 – 2018            Career break - Maternity leave

2012                        Research stay, MRC-University of Glasgow Centre for Virus Research, Laboratory of Arboviruses of

                                Prof Alain Kohl, Glasgow, Great Britain (3 months)

2008 – 2020            R&D Scientist, Institute of Parasitology, Biology Centre, Czech Academy of Sciences,

                                the Laboratory of Molecular Biology of Vectors and Parasites

Education

2009 – present        Ph.D. Studies in Parasitology, Faculty of Science, University of South Bohemia in České Budějovice

2009                        MSc. In Parasitology, Faculty of Science, University of South Bohemia in České Budějovice

2007                        BSc. in Biology, Faculty of Science, University of South Bohemia in České Budějovice

Selinger M., Věchtová P., Tykalová H., Ošlejšková P., Rumlová M., Štěrba J., Grubhoffer L., (2022). Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors. Computational and Structural Biotechnology Journal, 20: 2759-2777. DOI 10.1016/j.csbj.2022.05.052

Helmová R., Hönig V., Tykalová H., Palus M., Bell-Sakyi L., Grubhoffer L. (2020). Tick-borne encephalitis virus adaptation in different host environments and existence of quasispecies. Viruses 12(8):902. DOI: 10.3390/v12080902

Selinger M. et Tykalová H.; Štěrba J.; Věchtová P.; Vavrušková Z.; Lieskovská J.; Kohl A.; Schnettler E.; Grubhoffer L. (2019) Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. PLoS neglected tropical diseases 13(9): e0007745. DOI: 10.1371/journal.pntd.0007745

Černý J., Selinger M., Palus M., Vavrušková Z., Tykalová H., Bell-Sakyi L., Štěrba J., Grubhoffer L., Růžek D. (2016). Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells. Virus Genes 52(3):309-16. DOI: 10.1007/s11262-015-1273-y

Weisheit, S., Villar, M., Tykalová, H., Popara, M., Loecherbach, J., Watson, M., Růžek, D., Grubhoffer, L.; de la Fuente, J., Fazakerley, J.K., Bell-Sakyi, L. (2015) Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis. Parasites & Vectors 8:599. DOI: 10.1186/s13071-015-1210-x

Hönig V., Švec P., Halas P., Vavrušková Z., Tykalová H., Kilian P., Vetišková V., Dorňáková V., Štěrbová J., Šimonová Z., Erhart J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L. (2015). Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks and Tick-Borne Diseases 6(5): 559-67. DOI: 10.1016/j.ttbdis.2015.04.010

Schnettler E., Tykalová H., Watson M., Sharma M., Sterken M.G., Obbard D.J., Lewis S.H., McFarlane M., Bell-Sakyi L., Barry G., Weisheit S., Best S.M., Kuhn R.J., Pijlman G.P., Chase-Topping M.E., Gould E.A., Grubhoffer L., Fazakerley J.K., Kohl A. (2014) Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses. Nucleic Acids Research 42(14): 9436-46. DOI: 10.1093/nar/gku657

Ahantarig, A., Růžek, D., Vancová, M., Janowitz, A., Šťastná, H., Tesařová, M., Grubhoffer, L. (2009). Tick-borne encephalitis virus infection of cultured mouse macrophages. Intervirology 52(5): 283-90. DOI: 10.1159/000235741

Růžek D., Šťastná H., Kopecký J., Golovjova I., Grubhoffer L. (2007). Rapid subtyping of tick-borne encephalitis virus-isolates using multiplex RT-PCR. Journal of Virological Methods 144(1-2): 133-7.          DOI: 10.1016/s1386-6532(06)80817-6          

Number of publications: 9, H-index: 6, Citations w/o self-citations (Scopus): 209

Conference abstracts published in peer-reviewed journals

Grubhoffer L., Selinger M., Tykalová H., Schnettler E., Štěrba J. (2019). Tick-borne encephalitis virus inhibits production of ribosomal RNA in human cells of neuronal origin. FEBS OPEN BIO 9, 9 (suppl. 1): 158-159.

Selinger M., Tykalová H., Vavrušková Z., Schnettler E., Kohl A., Štěrba J., Grubhoffer, L., (2018). Tick-borne encephalitis virus induces translational shut-off in human cells of neural origin. FEBS OPEN BIO 8, 8 (suppl. 1): 199-199.

Růžek D., Šťastná H., Kopecký J., Grubhoffer L. (2006). Multiplex RT-PCR for detection and subtyping of tick-borne encephalitis virus. Journal of Clinical Virology 36 (suppl. 2): S32.

P.I.: 2001 043/2011/P The role of viperin in cells infected with tick-borne encephalitis virus, The Grant Agency of the University of South Bohemia.

Research team member of 6 projects             GAČR (4), MŠMT (2)